Biological Physics

Supramolecular Structures Lab
Lösche/Heinrich Group

Lab publications

2015

F. Heinrich, S. Chakravarthy, H. Nanda, A. Papa, P.P. Pandolfi, A.H. Ross, R. K. Harishchandra, A. Gericke, M. Lösche, The PTEN tumor suppressor forms homodimers in solution, Structure (2015), in press.
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T. L. Yap, Z. Jiang, F. Heinrich, J. M. Gruschus, C .M. Pfefferkorn, M. Barros, J .E. Curtis, E. Sidransky, J. C. Lee, Structural features of membrane-bound glucocerebrosidase and α-synuclein probed by neutron reflectometry and fluorescence spectroscopy, J. Biol. Chem. 290 (2015), 744 - 754.
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H. Nanda, F. Heinrich, and M. Lösche, Membrane association of the PTEN tumor suppressor: Neutron scattering and MD simulations reveal the structure of protein-membrane complexes, Methods 77–78 (2015), 136 - 146.
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2014

A. Kalinowski, P. N. Yaron, Z. Qin, S. Shenoy, M. J. Buehler, M. Lösche, and K. N. Dahl, Interfacial binding and aggregation of lamin A tail domains associated with Hutchinson–Gilford progeria syndrome, Biophys. Chem. 195 (2014), 43 - 38.
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A. Benedetto, F. Heinrich, M. A. Gonzalez, G. Fragneto, E. Watkins, P. Ballone, Structure and Stability of Phospholipid Bilayers Hydrated by a Room-Temperature Ionic Liquid/Water Solution: A Neutron Reflectometry Study, J. Phys. Chem. B 118 (2014), 12192 - 12206.

R. Pulido, J. T. Barata, A. Carracedo, V. J. Sid, I. D. Chin-Sang, V. Davé, J. den Hertog, P. Devreotes, B. Eickholt, C. Eng, F. B. Furnari, M. M. Georgescu, A. Gericke, B. Hopkins, X. Jiang, S. R. Lee, M. Lösche, P. Malaney, X. Matias-Guiu, M. Molina, P. P. Pandolfi, R. Parsons, P. Pinton, C. Rivas, R. M. Rocha, M. S. Rodriguez, A. H. Ross, A. Serrano, V. Stambolic, B. Stiles, A. Suzuki, S. S. Tan, N. K. Tonks, L. C. Trotman, N. Wolff, R. Woscholski, H. Wu, and N. R. Leslie, A unified nomenclature and amino acid numbering for human PTEN, Sci. Signal. 7 (2014), pe15.

F. Heinrich and M. Lösche, Zooming in on disordered systems: Neutron reflection studies of proteins associated with fluid membranes, Biochim. Biophys. Acta 1838 (2014), 2341 - 2349.
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F. Heinrich, H. Nanda, H. Z. Goh, C. Bachert, M. Lösche, and A. D. Linstedt, Myristoylation restricts orientation of the GRASP domain on membranes and promotes membrane tethering, J. Biol. Chem. 289 (2014), 9683 - 9691.
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2013

A. Gericke, N. R. Leslie, M. Lösche, and A. H. Ross, PI(4,5)P2-mediated cell signaling: Emerging principles and PTEN as a paradigm for regulatory mechanism, Ch. 6 in: Advances in Experimental Medicine and Biology, Vol. 991, Lipid-Mediated Membrane Targeting of Proteins, Ed.: D. G. S. Capelluto (Springer, New York, 2013), 85 - 104.
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R. Budvytyte, M. Mickevicius, D. J. Vanderah, F. Heinrich and G. Valincius, Modification of tethered bilayers by phospholipid exchange with vesicles. Langmuir 29 (2013), 4320 - 4327.

A. Kalinowski, Z. Qin, K. Coffey, R. Kodali, M. J. Buehler, M. Lösche, K. N. Dahl, Calcium causes a conformational change in lamin A tail domain that promotes farnesyl-mediated membrane association, Biophys. J. 104 (2013), 2246 - 2253.
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R. Budvytyte, G. Valincius, G. Niaura, V. Voiciuk, M. Mickevicius, H. Chapman, H.-Z. Goh, P. Shekhar, F. Heinrich, S. Shenoy, M. Lösche, and D. J. Vanderah, Structure and properties of tethered bilayer lipid membranes with unsaturated anchor molecules, Langmuir 29 (2013), 8645 - 8656.
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2012

S. Shenoy, H. Nanda and M. Lösche, Membrane association of the PTEN tumor suppressor: Electrostatic interaction with phosphatidylserine-containing bilayers and regulatory role of the C-terminal tail, J. Struct. Biol. 180 (2012), 394 - 408.
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Goh H. Z., C. Tan, M. Deserno, F. Lanni, and M. Lösche, Hemifusion of giant unilamellar vesicles with planar hydrophobic surfaces: A fluorescence microscopy study, Soft Matter 8 (2012), 10877 - 10886.
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C. M. Pfefferkorn, F. Heinrich, A. J. Sodt, A. S. Maltsev, R. W. Pastor and J. C. Lee, Depth of α-synuclein in a bilayer determined by fluorescence, neutron reflectometry, and computation, Biophys. J. 102 (2012), 613 - 621.

S. S. Pai, F. Heinrich, A. L. Canady, T. M. Przybycien and R. D. Tilton, Coverage-dependent morphology of PEGylated lysozyme layers adsorbed on silica, J. Colloid Interf. Sci. 370 (2012), 170 - 175.

B. J. Kirby, P. A. Kienzle, B. B. Maranville, N. F. Berk, J. Krycka, F. Heinrich and C. F. Majkrzak, Phase-sensitive specular neutron reflectometry for imaging the nanometer scale composition depth profile of thin-film materials, Curr. Opin. Colloid Interf. Sci. 17 (2012), 44 - 53.

G. J. Hardy, R. Nayak, S. Munir Alam, J. G. Shapter, F. Heinrich and S. Zauscher, Biomimetic supported lipid bilayers with high cholesterol content, J. Mater. Chem. 22 (2012), 19506 - 19513.

S. Shenoy, P. Shekhar, F. Heinrich, M.-C. Daou, A. Gericke, A. H. Ross, and M. Lösche, Membrane association of the PTEN tumor suppressor: Molecular details of the protein-membrane complex from SPR binding studies and neutron reflection, PLoS One 7 (2012), e32591.
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M. I. Lioudyno, M. Broccio, Y. Sokolov, S. Rasool, J. Wu, M. T. Alkire, V. Liu, J. A. Kozak, P. R. Dennison, C. G. Glabe, M. Lösche, and J. E. Hall, Effect of synthetic Aβ peptide oligomers and fluorinated solvents on Kv1.3 channel properties and membrane conductance, PLoS One 7 (2012), e35090.
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2011

P. N. Yaron, B. D. Holt, P. A. Short, M. Lösche, M. F. Islam, and K. N. Dahl, Single wall carbon nanotubes enter cells by endocytosis and not membrane penetration, J. Nanobiotechn. 9 (2011), 45-1 - 15.
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S. A. K. Datta, F. Heinrich, S. Raghunandan, S. Krueger, J. E. Curtis, A. Rein and H. Nanda, HIV-1 Gag extension: Conformational changes require simultaneous interaction with membrane and nucleic acid, J. Mol. Biol. 406 (2011), 205 - 214.

P. Shekhar, H. Nanda, M Lösche, and F. Heinrich, Continuous distribution model for the investigation of complex molecular architectures near interfaces with scattering techniques, J. Appl. Phys. 110 (2011), 102216-1 - 12.
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C. F. Majkrzak, E. Carpenter, F. Heinrich and N. F. Berk, When beauty is only skin deep; optimizing the sensitivity of specular neutron reflectivity for probing structure beneath the surface of thin films, J. Appl. Phys. 110 (2011), 102212-1 - 14.

2010

H. Nanda, S. Datta, F. Heinrich, M. Lösche, J. E. Curtis, A. Rein, S. Krueger, Electrostatic interactions and binding orientation of HIV-1 matrix, studied by neutron reflectivity, Biophys. J. 99 (2010), 2516 - 2524. BJ Website / download paper / download supplement

P. Cizas, R. Budvytyte, R. Morkuniene, R. Moldovan, M. Broccio, M. Lösche, G. Niaura, G. Valincius, and V. Borutaite, Size-dependent neurotoxicity of β-amyloid oligomers, Arch. Biochem. Biophys. 496 (2010), 84 - 92. ABB Website / download paper

S. Shenoy, R. Moldovan, J. Fitzpatrick, D. J. Vanderah, M. Deserno, and M. Lösche, In-plane homogeneity and lipid dynamics in tethered Bilayer Lipid Membranes (tBLMs), Soft Matter 6 (2010), 1263 - 1274. Soft Matter Website / download paper

M. B. Smith, D. J. McGillivray, J. Genzer, M. Lösche, and P. K. Kilpatrick, Neutron reflectometry of supported hybrid bilayers with inserted peptide, Soft Matter 6 (2010), 862 - 865. Soft Matter Website / download paper

select pre-2010

S. A. Holt, A. P. Le Brun, C. F. Majkrzak, D. J. McGillivray, F. Heinrich, M. Lösche and J. H. Lakey, An ion-channel-containing membrane: Structural determination by magnetic contrast neutron reflectometry, Soft Matter 5 (2009), 2576 - 2586.
Soft Matter Website / download paper / download supplement

F. Heinrich, T. Ng, D. J. Vanderah, P. Shekhar, M. Mihailescu, H. Nanda, and M. Lösche, A new lipid anchor for sparsely-tethered bilayer lipid membranes, Langmuir 25 (2009), 4219 - 4229. Langmuir Website / download paper

D. J. McGillivray, G. Valincius, F. Heinrich, J. W. F. Robertson, D. J. Vanderah, W. Febo-Ayala, I. Ignatjev, M. Lösche, and J. J. Kasianowicz, Structure of functional Staphylococcus aureus α-hemolysin channels in tethered bilayer lipid membranes, Biophys. J. 96 (2009), 1547 - 1553. BJ Website / download paper / download supplement

G. Valincius, F. Heinrich, R. Budvytyte, D. J. Vanderah, D. J. McGillivray, Y. Sokolov, J. E. Hall, and M. Lösche, Soluble amyloid β oligomers affect dielectric membrane properties by bilayer insertion and domain formation: Implications for cell toxicity, Biophys. J. 95 (2008), 4845 - 4861. BJ Website / download paper / download supplement

W. Knoll, R. Naumann, M. Friedrich, J. W. F. Robertson, M. Lösche, F. Heinrich, D. J. McGil-livray, B. Schuster, P. C. Gufler, D. Pum, and U. B. Sleytr, Solid supported lipid membranes: New concepts for the biomimetic functionalization of solid surfaces, Biointerphases 3 (2008), FA125 - FA135. download paper

I. K. Vockenroth, C. Ohm, J. W. F. Robertson, D. J. McGillivray, M. Lösche, and I. Köper, Stable insulating tethered bilayer lipid membranes, Biointerphases 3 (2008), FA68 – FA73. download paper

D. J. McGillivray, G. Valincius, D. J. Vanderah, W. Febo-Ayala, J. T. Woodward, F. Heinrich, J. J. Kasianowicz, and M. Lösche, Molecular-scale structural and functional characterization of sparsely tethered bilayer membranes, Biointerphases 2 (2007), 21 – 33. download paper / download supplement

J. A. Dura, D. J. Pierce, C. F. Majkrzak, N. Maliszewskyj, D. J. McGillivray, M. Lösche, K. V. O’Donovan, M. Michailescu, U. A. Perez-Salas, D. L. Worcester, and S. H. White, AND/R: Advanced neutron diffractometer/reflectometer for investigation of thin films and multilayers for the life sciences, Rev. Sci. Instrum. 77 (2006), 074301.

J. Pittler, W. Bu, D. Vaknin, A. Travesset, D. J. McGillivray, and M. Lösche, Charge inversion at minute electrolyte concentrations, Phys. Rev. Lett. 97 (2006), 046102.

G. Valincius, D. J. McGillivray, W. Febo-Ayala, D. J. Vanderah, J. J. Kasianowicz, and M. Lösche, Enzyme activity to augment the characterization of tethered bilayer membranes, J. Phys. Chem. B  110 (2006), 10213 – 10216.

M. Dyck and M. Lösche, Interaction of the neurotransmitter, neuropeptide Y, with phospholipid membranes: Film balance and fluorescence microscopy studies, J. Phys. Chem. B  110 (2006), 22143 – 22151.

M. Dyck, A. Kerth, A. Blume, and M. Lösche, Interaction of the neurotransmitter, neuropeptide Y, with phospholipid membranes: Infrared spectroscopic characterization at the air/water inter­face, J. Phys. Chem. B  110, 22152 – 22159.

T. Gutberlet and M. Lösche, Complex biomimetic structures at fluid surfaces and sold/liquid interfaces, chapt. 14 in: Neutron Scattering in Biology, J. Fitter, T. Gutberlet, J. Katsaras, Eds. (Springer, New York, 2006), 283 – 306.

M. Dyck, P. Krüger and M. Lösche, Headgroup organization and hydration of methylated phosphatidylethanolamines in Langmuir monolayers, Phys. Chem. Chem. Phys. 7 (2005), 150 – 156.

P. Krüger and M. Lösche, Characterization of Floating Surface Layers of Lipids and Lipopolymers by Surface-Sensitive Scattering, in: Lecture Notes in Physics, Vol. 634, Eds.: R. Haberlandt, D. Michel, A. Pöppl and R. Stannarius, Springer, New York (2004), 395 – 438.

T. M. Fischer and M. Lösche, Pattern Formation in Langmuir Monolayers Due to Long-Range Electrostatic Interactions, in: Lecture Notes in Physics, Vol. 634, Eds.: R. Haberlandt, D. Michel, A. Pöppl and R. Stannarius, Springer, New York (2004), 383 – 394.

D. Vaknin, P. Krüger and M. Lösche, Anomalous x-ray reflectivity characterization of ion distribution at biomimetic membranes, Phys. Rev. Lett. 90 (2003), 178102.

M. Ferreira, P. Dynarowicz-Latka, J. Miñones Jr., W. Caetano, K. Kita, M. Schalke, M. Lösche, and O. N. Oliveira Jr., On the origin of the plateau in surface-pressure isotherms of aromatic carboxylic acids, J. Phys. Chem. B 106 (2002), 10395 – 10400.

M. Lösche, Surface-sensitive X-ray and neutron scattering characterization of planar lipid model membranes and lipid/peptide interactions. In: Current Topics in Membranes, Vol. 52, Ch. 5, Eds.: S. A. Simon and T. J. McIntosh, Academic Press, San Diego (2002), 115 – 160.

P. Esquinazi, A. Setzer, R. Höhne, C. Semmelhack, Y. Kopelevich, D. Spemann, T. Butz, B. Kohlstrunk, and M. Lösche, Weak ferromagnetism in oriented graphite samples, Phys. Rev. B 66 (2002), 024429.

P. Krüger, J. E. Baatz, R. A. Dluhy, and M. Lösche, Effect of hydrophobic surfactant peptides SP–B and SP–C on binary phospholipid monolayers. II. Molecular machinery at the air/water interface, Biophys. Chem. 99 (2002), 209 – 228.

M. Weygand, K. Kjaer, P. B. Howes, B. Wetzer, D. Pum, U. B. Sleytr, and M. Lösche, Structural reorganization of phospholipid headgroups upon recrystallization of an S-layer lattice, J. Phys. Chem. B 106 (2002), 5793 – 5799.

R. Stannarius, R. Köhler, U. Dietrich, M. Lösche, C. Tolksdorf, and R. Zentel, Structure and elastic properties of smectic liquid crystalline elastomer samples, Phys. Rev. E, 65 (2002), 041707.

Z. Khattari, P. Heinig, S. Wurlitzer, P. Steffen, M. Lösche, and T. M. Fischer, Wetting in asymmetric quasi-2D systems, Langmuir 18 (2002), 2273 – 2279.

M. Lösche and P. Krüger, Morphology of Langmuir monolayer phases, in: Lecture Notes in Physics, Vol. 600, Eds.: K. R. Mecke and D. Stoyan, Springer, New York (2002), 159 – 178.

R.-J. Zhang, P. Krüger, B. Kohlstrunk, and M. Lösche, Self-organized lateral patterning of a rare earth complex and stearic acid in Langmuir films, ChemPhysChem 2 (2001), 452 – 457.

W. Caetano, M. Ferreira, M. Tabak, M.I. Mosquera Sanchez, O.N. Oliveira Jr., P. Krüger, M. Schalke, and M. Lösche, Cooperativity of phospholipid reorganization upon interaction of dipyridamole with surface monolayers on water, Biophys. Chem. 91 (2001), 21 – 35.

W. Lehmann, H. Skupin, E. Gebhard, C. Tolksdorf, R. Zentel, P. Krüger, M. Lösche, and F. Kremer, Giant lateral electrostriction in ferroelectric liquid crystalline elastomers, Nature 410 (2001), 447 – 450.

E. Politsch, G. Cevc, A. Wurlitzer, and M. Lösche, Conformation of polymer brushes at aqueous surfaces determined with x-ray and neutron reflectometry. I. Novel data evaluation procedure for polymers at interfaces, Macromolecules 34 (2001), 1328 – 1333.

A. Wurlitzer, E. Politsch, S. Hübner, P. Krüger, M. Weygand, K. Kjaer, P. Hommes, O. Nuyken, G. Cevc, and M. Lösche, Conformation of polymer brushes at aqueous surfaces determined with x-ray and neutron reflectometry. II. High density phase transition of lipopolyoxazolines, Macromolecules 34 (2001), 1334 – 1342.

P. Krüger, M. Schalke, J. Linderholm, and M. Lösche, Multipurpose x-ray reflectometer optimized for the characterization of organic surface films on aqueous subphases, Rev. Sci. Instrum. 72 (2001), 184 – 192.

M. Lösche and M. Schalke, Structural models of lipid surface monolayers from x-ray and neutron reflectivity measurements, Adv. Colloid Interf. Sci. 88 (2000), 243 – 274.

P. Krüger and M. Lösche, Molecular chirality and the domain shapes in lipid monolayers on aqueous surfaces, Phys. Rev. E 62 (2000), 7031 – 7043.

G. Marshall, E. Teer, C.M. Knobler, M. Schalke, and M. Lösche, Phase diagrams for monolayers of long-chain thioacetates, Colloids Surf. A 171 (2000), 41– 48.

T. Gutberlet, A. Wurlitzer, U. Dietrich, E. Politsch, G. Cevc, R. Steitz, and M. Lösche, Organisation of tethered polyoxazoline polymer brushes at the air/water interface, Physica B 283 (2000), 37 – 39.

A. Wurlitzer, E. Politsch, G. Cevc, T. Gutberlet, K. Kjaer, and M. Lösche, Neutron and x-ray reflection from surface monolayers of a lipopolyoxazoline, Physica B 276–278 (2000), 343 – 344.

M. Schalke, P. Krüger, M. Weygand, and M. Lösche, Submolecular organization of DMPA in surface monolayers: Beyond the two-layer model. Biochim. Biophys. Acta 1464 (2000) 113 – 126.

M. Weygand, M. Schalke, P.B. Howes, K. Kjaer, J. Friedmann, B. Wetzer, D. Pum, U.B. Sleytr, and M. Lösche, Coupling of protein sheet crystals (S-layers) to phospholipid monolayers. J. Mater. Chem. 10 (2000) 141 – 148.

V. Hartmann, M. Lösche, S.V. Mello, and O.N. Oliveira, Jr., UV/Vis and SFM studies on  Langmuir monolayers and Langmuir-Blodgett films of 16-mer aniline and o-pentadecylaniline blends. Mater. Sci. Engin. C. 8/9 (1999), 425 – 429.

P. Krüger, M. Schalke, Z. Wang, R.H. Notter, R.A. Dluhy, and M. Lösche, Effect of hydrophobic surfactant proteins SP–B and SP–C on binary phospholipid monolayers. I. Fluorescence and darkfield microscopy. Biophys. J. 77 (1999), 903 – 914.

S.A.W. Verclas, P.B. Howes, K. Kjaer, A. Wurlitzer, M. Weygand, G. Büldt, N.A. Dencher, and M. Lösche, Grazing incidence X-ray diffraction from a single layer of purple membrane at the air/water interface. J. Mol. Biol. 287 (1999), 837 – 843.

M. Weygand, B. Wetzer, D. Pum, U.B. Sleytr, K. Kjaer, P.B. Howes, and M. Lösche, Bacterial Surfacelayer protein coupling to lipid membranes: X-ray reflectivity and grazing incidence diffraction studies. Biophys. J. 76 (1999), 458 – 468.

M. Lösche, J. Schmitt, G. Decher, W.G. Bouwman, and K. Kjaer. Detailed structure of molecularly thin polyelectrolyte multilayer films on solid substrates as revealed from neutron reflectometry. Macromolecules 31 (1998), 8893 – 8906.

B. Wetzer, A. Pfandler, E. Györvary, D. Pum, M. Lösche, and U.B. Sleytr, S-layer reconstitution at phospholipid monolayers. Langmuir 14 (1998), 6899 – 6906.

C. Lautz, T.M. Fischer, M. Weygand, M. Lösche, P. Howes, and K. Kjaer, Determination of alkyl chain tilt angles in Langmuir monolayers: A comparison of Brewster angle autocorrelation spectroscopy and x-ray diffraction. J. Chem. Phys. 108 (1998), 4640 – 4646.

A. Diederich and M. Lösche, Novel biosensoric devices based on molecular protein hetero-multilayer films. Adv. Biophys. 34 (1997), 205 – 230.

M. Lösche, Protein monolayers at interfaces, Curr. Opinion Solid State Mater. Sci. 2 (1997), 546 – 556.

A. Diederich, C. Sponer, D. Pum, U.B. Sleytr, and M. Lösche, Reciprocal influence between the protein and lipid components of a lipid-protein membrane model. Colloids and Surfaces B: Biointerfaces 6 (1996), 335 – 346.